Enzyme Kinetics - Enzyme Concentration

Effect of Enzyme Concentration



The Experiments

The effect of enzyme concentration on the rate of enzyme action will be determined by performing four experiments:

1. One half ml of catalase solution mixed with two ml of H2O2 and 1.5 ml of water at 25oC.

2. One ml of catalase solution mixed with two ml of H2O2 and 1.0 ml of water at 25oC.

3. One and a half ml of catalase solution mixed with two ml of H2O2 and 0.5 ml of water at 25oC.

4. Two ml of catalase solution mixed with two ml of H2O2 and 0.0 ml of water at 25oC.

Notice that the only experimental variable being changed is the concentration of the enzyme being used. The water is added to maintain a constant volume in all experiments.


Results

Using the data below prepare a graph with enzyme concentration as the independent variable (x-axis) and rate of O2 formation in mls/sec as the dependent variable (y-axis). From this graph describe the effect of enzyme concentration on rate of reaction.

experiment
# 		enzyme solution
(mls) 		see results
1 0.5
2 1.0
3 1.5
4 2.0